H00005810-M04

antibody from Abnova Corporation

Targeting: RAD1 HRAD1, REC1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005810-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005810-M04, RRID:AB_1112079

Product name

RAD1 monoclonal antibody (M04), clone 1A12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RAD1.

Antigen sequence

MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIH
FREHATCFATKNGIKVTVENAKCVQANAFIQAGIF
QEFKVQEESVTFRINLTVLL


Isotype

IgG

Antibody clone number

1A12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody (M04), clone 1A12.Lane 1: RAD1 transfected lysate (Predicted MW: 31.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RAD1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol