LS-C142758

antibody from LSBio

Targeting: BHLHE40 BHLHB2, Clast5, DEC1, SHARP2, STRA13

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C142758

Provider

LSBio

Product name

BHLHB2 / DEC1 Antibody (C-Terminus, Biotin) LS-C142758

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Biotin

Storage

Store at 4°C. Do not freeze.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human DEC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 mcg for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-DEC1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and for IP at 3 mcg/mg lysate. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). This image was taken for the unconjugated form of this product. Other forms have not been tested.