Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B2222 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B2222, RRID:AB_2065357
- Product name
- IHC-plus™ BHLHB2 / DEC1 Antibody (C-Terminus) LS-B2222
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human DEC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 mcg for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-DEC1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and for IP at 3 mcg/mg lysate. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). This image was taken for the unconjugated form of this product. Other forms have not been tested.