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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-34938 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAK6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH. Predicted reactivity: Mouse (91%), Rat (91%), Rhesus Monkey (99%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PAK6 using 30 µg of A) NT2D1 (B) PC-3 (C) U87-MG and D) SK-N-SH lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PAK6 polyclonal antibody (Product # PA5-34938) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PAK6 Polyclonal Antibody (Product # PA5-34938). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK6 Polyclonal Antibody (Product # PA5-34938) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PAK6 Polyclonal Antibody detects PAK6 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. PAK6 Polyclonal Antibody (Product # PA5-34938) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PAK6 Polyclonal Antibody immunoprecipitates PAK6 protein in IP experiments. IP samples: PC-3 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of PAK6 protein by 4 µg PAK6 Polyclonal Antibody (Product # PA5-34938). 7.5 % SDS-PAGE. The immunoprecipitated PAK6 protein was detected by PAK6 Polyclonal Antibody (Product # PA5-34938) diluted at 1:500.
