PAB7902

antibody from Abnova Corporation

Targeting: PAK6 PAK5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB7902

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB7902, RRID:AB_1710773

Product name

PAK6 (phospho S165) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of PAK6.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Activation of p21-activated kinase 6 by MAP kinase kinase 6 and p38 MAP kinase.

Kaur R, Liu X, Gjoerup O, Zhang A, Yuan X, Balk SP, Schneider MC, Lu ML
The Journal of biological chemistry 2005 Feb 4;280(5):3323-30

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Mechanism of p21-activated kinase 6-mediated inhibition of androgen receptor signaling.

Schrantz N, da Silva Correia J, Fowler B, Ge Q, Sun Z, Bokoch GM
The Journal of biological chemistry 2004 Jan 16;279(3):1922-31

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AR and ER interaction with a p21-activated kinase (PAK6).

Lee SR, Ramos SM, Ko A, Masiello D, Swanson KD, Lu ML, Balk SP
Molecular endocrinology (Baltimore, Md.) 2002 Jan;16(1):85-99

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blots of human PAK6 recombinant protein phosphorylated by ERK2. The blot was exposed to lambda phosphatase (lane 2) then probed with PAK6 (phospho S165) polyclonal antibody (Cat # PAB7902) (lanes 1-4). The PAK6 antibody was used in the presence of PAK6 (Ser-165) antibody was used in the presence of phospho-PAK6 (Ser-165) peptide (lane 3) or a non-specific phospho-serine peptide (lane 4).