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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP53154PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53154PU-N, RRID:AB_11144458
- Product name
- anti PAK6 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 275-305 amino acids from the Central region of human PAK6
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of PAK6 Antibody (Center) Cat.-No AP53154PU-NÂ in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PAK6 Antibody (Center) Cat.-No AP53154PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with AP53154PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
