PAB28698

antibody from Abnova Corporation

Targeting: NSF SEC18, SKD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28698

Provider

Abnova Corporation

Product name

NSF polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NSF.

Antigen sequence

KVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQ
EDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNS
DRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIK
ICSPDK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate)Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)with NSF polyclonal antibody (Cat # PAB28698) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with NSF polyclonal antibody (Cat # PAB28698) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with NSF polyclonal antibody (Cat # PAB28698) shows cytoplasmic positivity in cells of seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry