Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-32156 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PSF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSF1 Antibody [NBP2-32156] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSF1 Antibody [NBP2-32156] - Detection of Human PSF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PSF1 Antibody [NBP2-32156] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated PSF1, NBP2-32156 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PSF1 Antibody [NBP2-32156] - Detection of Human PSF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSF1 antibody NBP2-32156 used for IP at 3 ug per reaction. PSF1 was also immunoprecipitated by rabbit anti-PSF1 antibody NBP2-30365. For blotting immunoprecipitated PSF1, NBP2-32156 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
