Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-82166 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82166, RRID:AB_11006469
- Product name
- Rabbit Polyclonal NPLOC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human NPLOC4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4.
DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage.
Skrott Z, Mistrik M, Andersen KK, Friis S, Majera D, Gursky J, Ozdian T, Bartkova J, Turi Z, Moudry P, Kraus M, Michalova M, Vaclavkova J, Dzubak P, Vrobel I, Pouckova P, Sedlacek J, Miklovicova A, Kutt A, Li J, Mattova J, Driessen C, Dou QP, Olsen J, Hajduch M, Cvek B, Deshaies RJ, Bartek J
Nature 2017 Dec 14;552(7684):194-199
Nature 2017 Dec 14;552(7684):194-199
DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage.
Davis EJ, Lachaud C, Appleton P, Macartney TJ, Näthke I, Rouse J
Nature structural & molecular biology 2012 Nov;19(11):1093-100
Nature structural & molecular biology 2012 Nov;19(11):1093-100
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis in human cell line A-549.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NPLOC4 Antibody [NBP1-82166] - Whole cell lysate of PC12 using antibody against nuclear protein NPLOC4. Samples were run as triplicates. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis using Anti-NPLOC4 antibody NBP1-82166 (A) shows similar pattern to independent antibody NBP1-82165 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.