Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF4499 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PTK7/CCK4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse and rat PTK7/CCK4 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Downstream signaling and genome-wide regulatory effects of PTK7 pseudokinase and its proteolytic fragments in cancer cells.
Protein-tyrosine pseudokinase 7 (PTK7) directs cancer cell motility and metastasis.
Insights into ectodomain shedding and processing of protein-tyrosine pseudokinase 7 (PTK7).
Flt-1 regulates vascular endothelial cell migration via a protein tyrosine kinase-7-dependent pathway.
Potential relation of aberrant proteolysis of human protein tyrosine kinase 7 (PTK7) chuzhoi by membrane type 1 matrix metalloproteinase (MT1-MMP) to congenital defects.
Golubkov VS, Strongin AY
Cell communication and signaling : CCS 2014 Mar 11;12:15
Cell communication and signaling : CCS 2014 Mar 11;12:15
Protein-tyrosine pseudokinase 7 (PTK7) directs cancer cell motility and metastasis.
Golubkov VS, Prigozhina NL, Zhang Y, Stoletov K, Lewis JD, Schwartz PE, Hoffman RM, Strongin AY
The Journal of biological chemistry 2014 Aug 29;289(35):24238-49
The Journal of biological chemistry 2014 Aug 29;289(35):24238-49
Insights into ectodomain shedding and processing of protein-tyrosine pseudokinase 7 (PTK7).
Golubkov VS, Strongin AY
The Journal of biological chemistry 2012 Dec 7;287(50):42009-18
The Journal of biological chemistry 2012 Dec 7;287(50):42009-18
Flt-1 regulates vascular endothelial cell migration via a protein tyrosine kinase-7-dependent pathway.
Lee HK, Chauhan SK, Kay E, Dana R
Blood 2011 May 26;117(21):5762-71
Blood 2011 May 26;117(21):5762-71
Potential relation of aberrant proteolysis of human protein tyrosine kinase 7 (PTK7) chuzhoi by membrane type 1 matrix metalloproteinase (MT1-MMP) to congenital defects.
Golubkov VS, Aleshin AE, Strongin AY
The Journal of biological chemistry 2011 Jun 10;286(23):20970-6
The Journal of biological chemistry 2011 Jun 10;286(23):20970-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat PTK7/CCK4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human PTK7/CCK4 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 171 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.