HPA003222

antibody from Atlas Antibodies

Targeting: PTK7 CCK4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA003222

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003222, RRID:AB_1079713

Product name

Anti-PTK7

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TAIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVY
WLLDGAPVQDTERRFAQGSSLSFAAVDRLQDSGTF
QCVARDDVTGEEARSANASFNIKWIEAGPVVLKHP
ASEAEIQPQTQVTLRCHIDGHPRPTYQWFR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines Caco-2 and A-549 using Anti-PTK7 antibody. Corresponding PTK7 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RH-30.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Sample type

HUMAN