Explore
Validate
LearnAB-32-NA
antibody from R&D Systems
Targeting: FGF1
AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AB-32-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Bovine FGF acidic/FGF1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects bovine FGF acidic/FGF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize both bovine FGF acidic/FGF1 and recombinant human (rh) FGF acidic/FGF1. In direct ELISAs, no cross-reactivity with rhFGF-basic, rhFGF-4, -5, -6, -7 or -9 is observed. Neutralizes the biological activity of bovine FGF acidic/FGF1 and will also neutralize the biological activity of rhFGF acidic/FGF1, although 5 times the amount of IgG is required.
- Reactivity
- Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters.
Caron A, Michelet S, Caron A, Sordello S, Ivanov MA, Delaère P, Branellec D, Schwartz B, Emmanuel F
The journal of gene medicine 2004 Sep;6(9):1033-45
The journal of gene medicine 2004 Sep;6(9):1033-45
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF acidic/FGF1 and Neutral-ization by Bovine FGF acidic/FGF1 Antibody. Bovine FGF acidic/FGF1 (Catalog # 132-FA) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF acidic/FGF1 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Bovine FGF acidic/FGF1 Polyclonal Antibody (Catalog # AB-32-NA). The ND50 is typically 1-3 µg/mL.
