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LearnH00002246-M02
antibody from Novus Biologicals
Targeting: FGF1
AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00002246-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002246-M02, RRID:AB_669197
- Product name
- Mouse Monoclonal FGF acidic/FGF1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. fibroblast growth factor 1 (acidic)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Co-expression of podoplanin and fibroblast growth factor 1 predicts poor prognosis in patients with lung squamous cell carcinoma.
Repression of the DCL2 and DCL4 genes in Nicotiana benthamiana plants for the transient expression of recombinant proteins.
A simple agroinfiltration method for transient gene expression in plant leaf discs.
Li J, Chen H, Li X, Wang L, Gao A, Zhang P, Lin W, Gao W, Yang D, Guo X, Liu J, Dang Q, Sun Y
Molecular medicine reports 2017 Aug;16(2):1643-1652
Molecular medicine reports 2017 Aug;16(2):1643-1652
Repression of the DCL2 and DCL4 genes in Nicotiana benthamiana plants for the transient expression of recombinant proteins.
Matsuo K, Matsumura T
Journal of bioscience and bioengineering 2017 Aug;124(2):215-220
Journal of bioscience and bioengineering 2017 Aug;124(2):215-220
A simple agroinfiltration method for transient gene expression in plant leaf discs.
Matsuo K, Fukuzawa N, Matsumura T
Journal of bioscience and bioengineering 2016 Sep;122(3):351-6
Journal of bioscience and bioengineering 2016 Sep;122(3):351-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of FGF1 expression in transfected 293T cell line by FGF1 monoclonal antibody (M02), clone 2E12.Lane 1: FGF1 transfected lysate(17.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: FGF acidic/FGF1 Antibody (2E12.) [H00002246-M02] - FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Detection limit for recombinant GST tagged FGF1 is approximately 10ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of monoclonal antibody to FGF1 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of FGF1 transfected lysate using anti-FGF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGF1 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of monoclonal antibody to FGF1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
