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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- TA319568 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal anti-HEF1 antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal anti-HEF1 antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NEDD9
- Antibody clone number
- 2G9
- Vial size
- 100 µg
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using monoclonal anti-HEF1 antibody antibody shows detection of a 115 kDa band corresponding to HEF1 in MCF7 lysate (arrowhead). Approximately 35 ug of lysate was loaded for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,000 dilution of anti-HEF1 antibody. Detection occurred using a 1:5,000 dilution of IRDye?800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers (indicated at left).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using monoclonal anti-HEF1 antibody (clone 2G9) shows detection of endogenous HEF1 present in various cell lines. Panel A shows detection using a 15 min exposure. Panel B is the same blot exposed for 2 min. The doublet represents p105 and p115 staining. The lower MW band represents p55. 3Y1 cells are derived from rat fibroblast cells. Mouse 3T3 cells are also reactive (not shown). To date no staining has been noted on CHO cells.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy using Monoclonal anti-HEF1 antibody (clone 2G9) shows detection of HEF1 localized at focal adhesion sites. The antibody was used at a 1:500 dilution with a 3-sec exposure time. Personal Communication. Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.
- Validation comment
- IF
