PAB20040

antibody from Abnova Corporation

Targeting: PRPF39 FLJ11128, FLJ20666

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20040

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20040, RRID:AB_10961679

Product name

PRPF39 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PRPF39.

Antigen sequence

DINWENEQGNLREVTAIYDRILGIPTQLYSHHFQR
FKEHVQNNLPRDLLTGEQFIQLRRELASVNGHSGD
DGPPGDDLPSGIEDITDPAKLITEIENMRHRIIEI
HQEMFNYNEHEVSKRWTFEEGIKRPYFHVKPLEKA
QL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PRPF39 polyclonal antibody (Cat # PAB20040) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with PRPF39 polyclonal antibody (Cat # PAB20040) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with PRPF39 polyclonal antibody (Cat # PAB20040) shows strong nuclear positivity in cells in seminiferus ducts at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)