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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX105447 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105447, RRID:AB_1951068
- Product name
- Optineurin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1.
Prabakaran T, Bodda C, Krapp C, Zhang BC, Christensen MH, Sun C, Reinert L, Cai Y, Jensen SB, Skouboe MK, Nyengaard JR, Thompson CB, Lebbink RJ, Sen GC, van Loo G, Nielsen R, Komatsu M, Nejsum LN, Jakobsen MR, Gyrd-Hansen M, Paludan SR
The EMBO journal 2018 Apr 13;37(8)
The EMBO journal 2018 Apr 13;37(8)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A:A431(GTX27909)B:H1299C:Hep G2 (GTX27900)10% SDS PAGEGTX105447 diluted at 1:2000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Optineurin(GTX105447) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Optineurin antibody immunoprecipitates Optineurin protein in IP experiments.IP samples: HepG2 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of Optineurin protein by 4 £gg Optineurin antibody (GTX105447)7.5 % SDS-PAGEThe immunoprecipitated Optineurin protein was detected by Optineurin antibody (GTX105447) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Optineurin(GTX105447) antibody at 1:500 dilution.
