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LearnPA5-51882
antibody from Invitrogen Antibodies
Targeting: OPTN
FIP-2, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-51882 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Optineurin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LELAEKALAS KQLQMDEMKQ TIAKQEEDLE TMTILRAQME VYCSDFHAER AAREKIHEEK EQLALQLAVL LKENDAFEDG GRQSLMEMQS RHGARTSDSD QQAYLVQRGA EDRDWRQQRN IPIHSCPKCG EVL
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Optineurin in human cell lines A-431 and HEK293 using a Optineurin Polyclonal Antibody (Product # PA5-51882). Corresponding OPTN RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Optineurin using Optineurin Polyclonal Antibody (Product # PA5-51882) (A) shows similar pattern to an independent Optineurin Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Optineurin was performed by loading 30 µg of HCT 116 (Lane 1), HCT 116 - Optineurin knockout (Lane 2) Whole cell extracts. The blot was probed with Anti-Optineurin Polyclonal Antibody Antibody (Product # PA5-51882,0.1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to Optineurin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Optineurin Polyclonal Antibody (Product # PA5-51882) and a 66 kDa band corresponding to Optineurin was observed across all the tested cell lines and tissues, except Mouse Eye, Mouse Liver and Rat Eye. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), HEK-293 (Lane 3), Mouse Retina (Lane 4), Mouse Eye (Lane 5), Mouse Liver (Lane 6), Mouse Kidney (Lane 7), Rat Retina (Lane 8), Rat Eye (Lane 9) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Optineurin in human cell line A-431 shows positivity in cytoplasm. Samples were probed using an Optineurin Polyclonal Antibody (Product # PA5-51882).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Optineurin in human cerebral cortex using an Optineurin Polyclonal Antibody (Product # PA5-51882) shows moderate cytoplasmic positivity in neuronal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Optineurin in human testis using an Optineurin Polyclonal Antibody (Product # PA5-51882) shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Optineurin in human rectum using an Optineurin Polyclonal Antibody (Product # PA5-51882) shows moderate cytoplasmic positivity in cells in granular layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Optineurin in human kidney using an Optineurin Polyclonal Antibody (Product # PA5-51882) shows weak cytoplasmic positivity in cells in tubules.
