TA503139

antibody from OriGene

Targeting: LMCD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503139

Provider

OriGene

Proper citation

OriGene Cat#TA503139, RRID:AB_11126614

Product name

LMCD1 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)

Antibody type

Monoclonal

Description

LMCD1 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)

Host

Mouse

Conjugate

Unconjugated

Epitope

LMCD1

Isotype

IgG

Antibody clone number

OTI1H5

Vial size

100 µl

Concentration

0.8 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (RC200062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-LMCD1 mouse monoclonal antibody (TA503139) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMCD1(RC200062).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LMCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503139)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200062 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LMCD1 antibody(TA503139), and then analyzed by flow cytometry.

Validation comment

FC