MA1-610

antibody from Invitrogen Antibodies

Targeting: PRLR

Provider product page for MA1-610

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [50]
Comments [0]
Validations
Immunocytochemistry [3]
Immunohistochemistry [2]
Other assay [4]

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Product number: MA1-610 - Provider product page
Provider: Invitrogen Antibodies
Product name: Prolactin Receptor Monoclonal Antibody (U5)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues. MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor. The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor. Reconstitute with 100 µL PBS.
Reactivity: Human, Mouse, Rat, Porcine, Rabbit
Host: Mouse
Isotype: IgG
Antibody clone number: U5
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

PRLR protein structure - MA1-610 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Prolactin Receptor using Prolactin Receptor Monoclonal Antibody (U5) (Product # MA1-610) shows staining in A2058 Cells. Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor (Product # MA1-610) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Prolactin Receptor using Prolactin Receptor Monoclonal Antibody (U5) (Product # MA1-610) shows staining in HepG2 Cells. Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor (Product # MA1-610) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Prolactin Receptor using Prolactin Receptor Monoclonal Antibody (U5) (Product # MA1-610) shows staining in MCF-7 Cells. Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Prolactin Receptor (Product # MA1-610) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1. Chronic PRL treatment increases PRL receptor expression in the hippocampus. a PRL levels were detected in serum by ELISA. Values represent mean +- SEM (Vh n = 8; PRL n = 7). Data were analysed by the Mann-Whitney U test (*** p < 0.001). b Western blot analysis of PRL R expression in protein extracts from the hippocampus. Long (PRL R L, 100 kDa) and short (PRL R S, 40 kDa) isoforms of PRL receptors were measured, and representative immunoblots are shown. GAPDH was included for normalization. Measurements represent mean +- SEM (Vh n = 8, PRL n = 8). Data were analysed by Student's t test (* p < 0.05; ** p < 0.01). c Immunofluorescent detection of PRL R . Hippocampal sections were processed for PRL R immunostaining and analysed by confocal microscopy. The arrows show PRL R expression. Scale bar, 10 mum. d Graphs showing relative fluorescence density analysis in different layers of CA1, CA3, and DG as indicated in the top panel. Values represent mean +- SEM (Vh n = 7; PRL n = 6). Data were analysed by Student's t test and Mann-Whitney U test (* p < 0.05; ** p < 0.01; *** p < 0.001). PRL, prolactin; SP, stratum pyramidale, SR, stratum radiatum; SL, stratum lacunosum; SG, stratum granulosum; SM, stratum moleculare.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Prolactin and prolactin receptor immunoreactivity in the neural retina from adult female baboons. The tissues correspond to adult baboon ID 14871. Confocal images of immuno-stained retina sections identified cells expressing PRL and PRLR. ( A ) PRL expression (red, 1st Ab: mouse anti-human PRL 1:100; goat anti-mouse IgG Cy5 1:400). Nuclei (blue) were counterstained with DAPI. ( B ) PRLR expression (green, 1st Ab: mouse anti-human PRLR 1:250; goat anti-mouse IgG-FITC 1:250), nuclei (blue). ( C ) Negative control for IgG Cy3 antibody has no primary antibody (mouse anti-human PRL 1:100). ( D ) PRL retina sections (5 mum-thick) at 25X magnification, scale-bar 20 mum. ( E ) PRLR sections at 25X magnification, scale-bar 20 mum. ( F ) Double-stained sections identified cells expressing PRL (red, 1st Ab: mouse anti-human PRL 1:100; goat anti-mouse IgG Cy5 1:4000), and PRLR (green, 1st Ab: mouse anti-human PRLR 1:250; goat anti-mouse IgG-FITC 1:250) at 25X, scale-bar 20 mum. GCL (ganglion cell layer), INL (inner nuclear layer), ONL (outer nuclear layer), IPL (inner plexiform layer), OPL (outer plexiform layer), and POS (photoreceptor outer segment).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Prolactin (PRL) and prolactin receptor (PRLR) immunoreactivity in the neural retina in baboon fetus. The tissues correspond to fetus baboon ID 12354. Confocal images of immuno-stained retina sections identified cells expressing PRL and PRLR. ( A ) PRL expression (red, 1st Ab: mouse anti-human PRL 1:100; goat anti-mouse IgG Cy5 1:4000) in the retina of baboon fetus. ( B ) Stained retina sections with DAPI to counterstain nuclei (blue) at 25X magnification, scale-bar 20 mum. ( C ) Merged images of stained retinal sections to show PRL expression and DAPI, at 25X magnification, scale-bar 20 mum. ( D ) Cells expressing PRLR (green, 1st Ab: mouse anti-human PRLR 1:250; goat anti-mouse IgG-FITC 1:250). In the magnification the expression of PRL is positive (white arrows indicate the PRL positive cells). ( E ) Retinal sections stained with DAPI (nuclei in blue) at 25X magnification, scale-bar 20 mum. ( F ) Merged images to show PRLR expression and DAPI, at 25X magnification, scale-bar 20 mum. In the magnification the expression of PRLR is positive (white arrows indicate the PRLR positive cells) GCL (ganglion cell layer), INL (inner nuclear layer), ONL (outer nuclear layer), IPL (inner plexiform layer), OPL (outer plexiform layer), RPE (retinal pigmented epitelium), and POS (photoreceptor outer segment).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 Immunocytochemical detection of PRL receptors in cumulus cells after 20 h maturation of bovine cumulus-enclosed oocytes. Specific localizations were detected by MA1-610 antibody and the red 3-amino-9-ethylcarbazole (AEC) chromophore. Nuclei were counterstained with hematoxylin. (A) Positive staining. Black arrows indicate PRL receptor-specific immunoreaction. (B) Negative control performed by omitting the primary antibody. Original magnification: x 400.