- MA1-611 - Invitrogen Antibodies PRLR antibody

Submitted references Prolactin secretory rhythm of mated rats induced by a single injection of oxytocin.

Egli M, Bertram R, Toporikova N, Sellix MT, Blanco W, Freeman ME
American journal of physiology. Endocrinology and metabolism 2006 Mar;290(3):E566-72

Hypothesis paper Brain talks with fat--evidence for a hypothalamic-pituitary-adipose axis?

Schäffler A, Binart N, Schölmerich J, Büchler C
Neuropeptides 2005 Aug;39(4):363-7

JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization.

Rui H, Lebrun JJ, Kirken RA, Kelly PA, Farrar WL
Endocrinology 1994 Oct;135(4):1299-306

Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations.

Gagnerault MC, Touraine P, Savino W, Kelly PA, Dardenne M
Journal of immunology (Baltimore, Md. : 1950) 1993 Jun 15;150(12):5673-81

Mutational analysis of the ligand-binding domain of the prolactin receptor.

Rozakis-Adcock M, Kelly PA
The Journal of biological chemistry 1991 Sep 5;266(25):16472-7

Identification and functional activity of prolactin receptors in thymic epithelial cells.

Dardenne M, Kelly PA, Bach JF, Savino W
Proceedings of the National Academy of Sciences of the United States of America 1991 Nov 1;88(21):9700-4

Characterization and applications of monoclonal antibodies to the prolactin receptor.

Okamura H, Zachwieja J, Raguet S, Kelly PA
Endocrinology 1989 May;124(5):2499-508

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of Prolactin Receptor in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody (Product # MA1-611) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Prolactin Receptor in H-4-II-E Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody (Product # MA1-611) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Prolactin Receptor in SW480 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody (Product # MA1-611) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemical staining of PRL receptor in NB2 cells using Product # MA1-611.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized Rat pituitary gland tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Prolactin Receptor monoclonal antibody (Product # MA1-611) at a dilution of 1:50 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

MA1-611

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