Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF1167 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Prolactin R Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Prolactin R in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse Prolactin R and recombinant rat Prolactin R is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P16471
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression of prolactin receptors in normal canine mammary tissue, canine mammary adenomas and mammary adenocarcinomas.
Luteal and placental function in the bitch: spatio-temporal changes in prolactin receptor (PRLr) expression at dioestrus, pregnancy and normal and induced parturition.
Immunoregulation of autocrine prolactin: suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown.
Michel E, Feldmann SK, Kowalewski MP, Bley CR, Boos A, Guscetti F, Reichler IM
BMC veterinary research 2012 May 30;8:72
BMC veterinary research 2012 May 30;8:72
Luteal and placental function in the bitch: spatio-temporal changes in prolactin receptor (PRLr) expression at dioestrus, pregnancy and normal and induced parturition.
Kowalewski MP, Michel E, Gram A, Boos A, Guscetti F, Hoffmann B, Aslan S, Reichler I
Reproductive biology and endocrinology : RB&E 2011 Aug 3;9:109
Reproductive biology and endocrinology : RB&E 2011 Aug 3;9:109
Immunoregulation of autocrine prolactin: suppressing the expression of costimulatory molecules and cytokines in T lymphocytes by prolactin receptor knockdown.
Xu D, Lin L, Lin X, Huang Z, Lei Z
Cellular immunology 2010;263(1):71-8
Cellular immunology 2010;263(1):71-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Prolactin R by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prolactin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1167) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin R at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.