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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-38021 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PGC1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- At least three isoforms of PPARGC1A are known to exist; this antibody only detects the longest isoform. PA5-38021 is predicted to react with pig and bovine based on 100% sequence homology. Recommended positive controls: Human liver tissue/lysate, mouse liver tissue.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499.
Induction of mitochondrial biogenesis protects against acetaminophen hepatotoxicity.
Nkx6.1 decline accompanies mitochondrial DNA reduction but subtle nucleoid size decrease in pancreatic islet β-cells of diabetic Goto Kakizaki rats.
Wu L, Ran L, Lang H, Zhou M, Yu L, Yi L, Zhu J, Liu L, Mi M
Nutrition & metabolism 2019;16:27
Nutrition & metabolism 2019;16:27
Induction of mitochondrial biogenesis protects against acetaminophen hepatotoxicity.
Du K, Ramachandran A, McGill MR, Mansouri A, Asselah T, Farhood A, Woolbright BL, Ding WX, Jaeschke H
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2017 Oct;108(Pt A):339-350
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2017 Oct;108(Pt A):339-350
Nkx6.1 decline accompanies mitochondrial DNA reduction but subtle nucleoid size decrease in pancreatic islet β-cells of diabetic Goto Kakizaki rats.
Špaček T, Pavluch V, Alán L, Capková N, Engstová H, Dlasková A, Berková Z, Saudek F, Ježek P
Scientific reports 2017 Nov 15;7(1):15674
Scientific reports 2017 Nov 15;7(1):15674
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PGC-1 alpha in mouse liver tissue lysate using a PGC-1 alpha polyclonal antibody (Product # PA5-38021) at a dilution of 1 µg/mL (A) and 2 µg/mL (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of Hep G2 (Lane 1), A-431 (Lane 2) and HeLa (Lane 3). The blot was probed with Anti- PGC1 alpha Polyclonal Antibody (Product # PA5-38021, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 90 kDa band corresponding to PGC1 alpha was detected across the cell lines tested. An uncharacterized band (*) around 60kDa was observed in all the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PGC-1 alpha in human liver tissue using a PGC-1 alpha polyclonal antibody (Product # PA5-38021) at a dilution of 20 µg/mL.
