NB100-60955

antibody from Novus Biologicals

Targeting: PPARGC1A PGC-1alpha, PGC1, PGC1A, PPARGC1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-60955

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-60955, RRID:AB_2166507

Product name

Goat Polyclonal PGC1 alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Goat

Antigen sequence

DGLFDDSEDESDK


Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Tetrabromobisphenol A-Induced Apoptosis in Neural Stem Cells Through Oxidative Stress and Mitochondrial Dysfunction.

Cho JH, Lee S, Jeon H, Kim AH, Lee W, Lee Y, Yang S, Yun J, Jung YS, Lee J
Neurotoxicity research 2020 Jun;38(1):74-85

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Extracellular Superoxide Dismutase Attenuates Renal Oxidative Stress Through the Activation of Adenosine Monophosphate-Activated Protein Kinase in Diabetic Nephropathy.

Hong YA, Lim JH, Kim MY, Kim Y, Park HS, Kim HW, Choi BS, Chang YS, Kim HW, Kim TY, Park CW
Antioxidants & redox signaling 2018 Jun 10;28(17):1543-1561

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Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury.

Zhang W, Zhong W, Sun Q, Sun X, Zhou Z
Scientific reports 2018 Jan 11;8(1):408

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Peroxisome proliferator-activated receptor gamma-coactivator-1 alpha coordinates sphingolipid metabolism, lipid raft composition and myelin protein synthesis.

Camacho A, Huang JK, Delint-Ramirez I, Yew Tan C, Fuller M, Lelliott CJ, Vidal-Puig A, Franklin RJ
The European journal of neuroscience 2013 Sep;38(5):2672-83

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Stat3-mediated activation of microRNA-23a suppresses gluconeogenesis in hepatocellular carcinoma by down-regulating glucose-6-phosphatase and peroxisome proliferator-activated receptor gamma, coactivator 1 alpha.

Wang B, Hsu SH, Frankel W, Ghoshal K, Jacob ST
Hepatology (Baltimore, Md.) 2012 Jul;56(1):186-97

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A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China.

Yuan Y, Guo-Qing P, Yan T, Hong-Lin Y, Gong-Hua H, Cai-Gao Z
Medical oncology (Northwood, London, England) 2012 Dec;29(4):2904-10

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PGC-1alpha regulates the neuromuscular junction program and ameliorates Duchenne muscular dystrophy.

Handschin C, Kobayashi YM, Chin S, Seale P, Campbell KP, Spiegelman BM
Genes & development 2007 Apr 1;21(7):770-83

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PGC1 alpha Antibody [NB100-60955] - (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PGC1 alpha Antibody [NB100-60955] - Staining of Rat Duodenum lysate with PGC1 alpha Antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) with PGC1 alpha Antibody, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.