Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-60955 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60955, RRID:AB_2166507
- Product name
- Goat Polyclonal PGC1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
DGLFDDSEDESDK
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Tetrabromobisphenol A-Induced Apoptosis in Neural Stem Cells Through Oxidative Stress and Mitochondrial Dysfunction.
Extracellular Superoxide Dismutase Attenuates Renal Oxidative Stress Through the Activation of Adenosine Monophosphate-Activated Protein Kinase in Diabetic Nephropathy.
Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury.
Peroxisome proliferator-activated receptor gamma-coactivator-1 alpha coordinates sphingolipid metabolism, lipid raft composition and myelin protein synthesis.
Stat3-mediated activation of microRNA-23a suppresses gluconeogenesis in hepatocellular carcinoma by down-regulating glucose-6-phosphatase and peroxisome proliferator-activated receptor gamma, coactivator 1 alpha.
A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China.
PGC-1alpha regulates the neuromuscular junction program and ameliorates Duchenne muscular dystrophy.
Cho JH, Lee S, Jeon H, Kim AH, Lee W, Lee Y, Yang S, Yun J, Jung YS, Lee J
Neurotoxicity research 2020 Jun;38(1):74-85
Neurotoxicity research 2020 Jun;38(1):74-85
Extracellular Superoxide Dismutase Attenuates Renal Oxidative Stress Through the Activation of Adenosine Monophosphate-Activated Protein Kinase in Diabetic Nephropathy.
Hong YA, Lim JH, Kim MY, Kim Y, Park HS, Kim HW, Choi BS, Chang YS, Kim HW, Kim TY, Park CW
Antioxidants & redox signaling 2018 Jun 10;28(17):1543-1561
Antioxidants & redox signaling 2018 Jun 10;28(17):1543-1561
Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury.
Zhang W, Zhong W, Sun Q, Sun X, Zhou Z
Scientific reports 2018 Jan 11;8(1):408
Scientific reports 2018 Jan 11;8(1):408
Peroxisome proliferator-activated receptor gamma-coactivator-1 alpha coordinates sphingolipid metabolism, lipid raft composition and myelin protein synthesis.
Camacho A, Huang JK, Delint-Ramirez I, Yew Tan C, Fuller M, Lelliott CJ, Vidal-Puig A, Franklin RJ
The European journal of neuroscience 2013 Sep;38(5):2672-83
The European journal of neuroscience 2013 Sep;38(5):2672-83
Stat3-mediated activation of microRNA-23a suppresses gluconeogenesis in hepatocellular carcinoma by down-regulating glucose-6-phosphatase and peroxisome proliferator-activated receptor gamma, coactivator 1 alpha.
Wang B, Hsu SH, Frankel W, Ghoshal K, Jacob ST
Hepatology (Baltimore, Md.) 2012 Jul;56(1):186-97
Hepatology (Baltimore, Md.) 2012 Jul;56(1):186-97
A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China.
Yuan Y, Guo-Qing P, Yan T, Hong-Lin Y, Gong-Hua H, Cai-Gao Z
Medical oncology (Northwood, London, England) 2012 Dec;29(4):2904-10
Medical oncology (Northwood, London, England) 2012 Dec;29(4):2904-10
PGC-1alpha regulates the neuromuscular junction program and ameliorates Duchenne muscular dystrophy.
Handschin C, Kobayashi YM, Chin S, Seale P, Campbell KP, Spiegelman BM
Genes & development 2007 Apr 1;21(7):770-83
Genes & development 2007 Apr 1;21(7):770-83
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGC1 alpha Antibody [NB100-60955] - (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGC1 alpha Antibody [NB100-60955] - Staining of Rat Duodenum lysate with PGC1 alpha Antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PGC1 alpha Antibody [NB100-60955] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) with PGC1 alpha Antibody, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.