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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Product number
- AF6650 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-PGC1 alpha (S571) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PGC1 alpha when phosphorylated at S571 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Coordinated regulation of hepatic FoxO1, PGC-1α and SREBP-1c facilitates insulin action and resistance.
Chloroquine and amodiaquine enhance AMPK phosphorylation and improve mitochondrial fragmentation in diabetic tubulopathy.
NADPH Oxidase 4 (Nox4) Suppresses Mitochondrial Biogenesis and Bioenergetics in Lung Fibroblasts via a Nuclear Factor Erythroid-derived 2-like 2 (Nrf2)-dependent Pathway.
Metabolic Reprogramming Is Required for Myofibroblast Contractility and Differentiation.
Sajan MP, Lee MC, Foufelle F, Sajan J, Cleland C, Farese RV
Cellular signalling 2018 Mar;43:62-70
Cellular signalling 2018 Mar;43:62-70
Chloroquine and amodiaquine enhance AMPK phosphorylation and improve mitochondrial fragmentation in diabetic tubulopathy.
Jeong HY, Kang JM, Jun HH, Kim DJ, Park SH, Sung MJ, Heo JH, Yang DH, Lee SH, Lee SY
Scientific reports 2018 Jun 8;8(1):8774
Scientific reports 2018 Jun 8;8(1):8774
NADPH Oxidase 4 (Nox4) Suppresses Mitochondrial Biogenesis and Bioenergetics in Lung Fibroblasts via a Nuclear Factor Erythroid-derived 2-like 2 (Nrf2)-dependent Pathway.
Bernard K, Logsdon NJ, Miguel V, Benavides GA, Zhang J, Carter AB, Darley-Usmar VM, Thannickal VJ
The Journal of biological chemistry 2017 Feb 17;292(7):3029-3038
The Journal of biological chemistry 2017 Feb 17;292(7):3029-3038
Metabolic Reprogramming Is Required for Myofibroblast Contractility and Differentiation.
Bernard K, Logsdon NJ, Ravi S, Xie N, Persons BP, Rangarajan S, Zmijewski JW, Mitra K, Liu G, Darley-Usmar VM, Thannickal VJ
The Journal of biological chemistry 2015 Oct 16;290(42):25427-38
The Journal of biological chemistry 2015 Oct 16;290(42):25427-38
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Phospho-PGC1 alpha (S571) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 10 nm Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-PGC1 alpha (S571) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6650) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PGC1 alpha (S571) at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
