Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-76619 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNMT3L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNMT3L. Samples were incubated with DNMT3L polyclonal antibody (Product # PA5-76619).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNMT3L. Samples were incubated with DNMT3L polyclonal antibody (Product # PA5-76619).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DNMT3L Polyclonal Antibody (Product # PA5-76619) and a 43 kDa band corresponding to DNMT3L was observed across the cell lines and tissues tested. Uncharacterized bands of ~ 110 kDa and ~30 kDa were observed in tissues. Modified whole cell extracts (1% SDS) (30 µg lysate) of BeWo (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), Caco-2 (Lane 4), PC-3 (Lane 5), SK-BR-3 (Lane 6), tissue extracts (30 µg lysate) of Mouse Testis (Lane 7), Rat Testis (Lane 8), Mouse Kidney (Lane 9) and Mouse Liver (Lane 10) were electrophoresed using Novex® NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution), using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).