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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-30570 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNMT3L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DNMT3L Polyclonal Antibody (Product # PA5-30570). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 10% SDS PAGE. DNMT3L Polyclonal Antibody (Product # PA5-30570) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNMT3L Polyclonal Antibody detects DNMT3L protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3L protein stained by DNMT3L Polyclonal Antibody (Product # PA5-30570) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNMT3L Polyclonal Antibody detects DNMT3L protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3L protein stained by DNMT3L Polyclonal Antibody (Product # PA5-30570) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Dnmt3L showing staining in the nucleus of NT2D1 cells. NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Dnmt3L polyclonal antibody (Product # PA5-30570) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
