R1572P

antibody from Acris Antibodies GmbH

Targeting: DNMT3L MGC1090

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

R1572P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1572P, RRID:AB_980481

Product name

anti DNMT3L (152-164)

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to aa 152-164 of Human DNMT3L.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.1 mg

Concentration

1.43 mg/ml (by UV absorbance at 280 nm) Format: This antibody is supplied as sterile filtered, imunoaffinity purified liquid Ig fraction in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoblotting: R1572P DNMT3L antibody was used at a 1/1,500 dilution to detect Human DNMT3L by Western blot (WB) after Immunoprecipitation (IP) using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 μg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al.(2000). For WB the antibody was used at 0.4 μg/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for WB only following Immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected Molecular weight of Human DNMT3L is 43.6 kDa.