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LearnH00056852-M01
antibody from Novus Biologicals
Targeting: RAD18
RNF73
Western blot ELISA Immunocytochemistry
Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- H00056852-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00056852-M01, RRID:AB_547545
- Product name
- Mouse Monoclonal RAD18 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RAD18 - RAD18 homolog (S. cerevisiae)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references USP1 Is Required for Replication Fork Protection in BRCA1-Deficient Tumors.
RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress.
HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.
The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks.
Identification of DNA repair pathways that affect the survival of ovarian cancer cells treated with a poly(ADP-ribose) polymerase inhibitor in a novel drug combination.
Proliferating cell nuclear antigen (PCNA)-binding protein C1orf124 is a regulator of translesion synthesis.
Elevated expression of Rad18 regulates melanoma cell proliferation.
Phosphorylation of human INO80 is involved in DNA damage tolerance.
Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks.
NBS1 recruits RAD18 via a RAD6-like domain and regulates Pol η-dependent translesion DNA synthesis.
Tumour suppressor ING1b maintains genomic stability upon replication stress.
Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links.
WRN participates in translesion synthesis pathway through interaction with NBS1.
Lim KS, Li H, Roberts EA, Gaudiano EF, Clairmont C, Sambel LA, Ponnienselvan K, Liu JC, Yang C, Kozono D, Parmar K, Yusufzai T, Zheng N, D'Andrea AD
Molecular cell 2018 Dec 20;72(6):925-941.e4
Molecular cell 2018 Dec 20;72(6):925-941.e4
RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress.
Kanu N, Zhang T, Burrell RA, Chakraborty A, Cronshaw J, DaCosta C, Grönroos E, Pemberton HN, Anderton E, Gonzalez L, Sabbioneda S, Ulrich HD, Swanton C, Behrens A
Oncogene 2016 Jul 28;35(30):4009-19
Oncogene 2016 Jul 28;35(30):4009-19
HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal.
Kile AC, Chavez DA, Bacal J, Eldirany S, Korzhnev DM, Bezsonova I, Eichman BF, Cimprich KA
Molecular cell 2015 Jun 18;58(6):1090-100
Molecular cell 2015 Jun 18;58(6):1090-100
The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks.
Sy SM, Jiang J, O WS, Deng Y, Huen MS
Nucleic acids research 2013 Oct;41(18):8572-80
Nucleic acids research 2013 Oct;41(18):8572-80
Identification of DNA repair pathways that affect the survival of ovarian cancer cells treated with a poly(ADP-ribose) polymerase inhibitor in a novel drug combination.
Huehls AM, Wagner JM, Huntoon CJ, Karnitz LM
Molecular pharmacology 2012 Oct;82(4):767-76
Molecular pharmacology 2012 Oct;82(4):767-76
Proliferating cell nuclear antigen (PCNA)-binding protein C1orf124 is a regulator of translesion synthesis.
Ghosal G, Leung JW, Nair BC, Fong KW, Chen J
The Journal of biological chemistry 2012 Oct 5;287(41):34225-33
The Journal of biological chemistry 2012 Oct 5;287(41):34225-33
Elevated expression of Rad18 regulates melanoma cell proliferation.
Wong RP, Aguissa-Touré AH, Wani AA, Khosravi S, Martinka M, Martinka M, Li G
Pigment cell & melanoma research 2012 Mar;25(2):213-8
Pigment cell & melanoma research 2012 Mar;25(2):213-8
Phosphorylation of human INO80 is involved in DNA damage tolerance.
Kato D, Waki M, Umezawa M, Aoki Y, Utsugi T, Ohtsu M, Murakami Y
Biochemical and biophysical research communications 2012 Jan 6;417(1):433-8
Biochemical and biophysical research communications 2012 Jan 6;417(1):433-8
Tandem protein interaction modules organize the ubiquitin-dependent response to DNA double-strand breaks.
Panier S, Ichijima Y, Fradet-Turcotte A, Leung CC, Kaustov L, Arrowsmith CH, Durocher D
Molecular cell 2012 Aug 10;47(3):383-95
Molecular cell 2012 Aug 10;47(3):383-95
NBS1 recruits RAD18 via a RAD6-like domain and regulates Pol η-dependent translesion DNA synthesis.
Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K
Molecular cell 2011 Sep 2;43(5):788-97
Molecular cell 2011 Sep 2;43(5):788-97
Tumour suppressor ING1b maintains genomic stability upon replication stress.
Wong RP, Lin H, Khosravi S, Piche B, Jafarnejad SM, Chen DW, Li G
Nucleic acids research 2011 May;39(9):3632-42
Nucleic acids research 2011 May;39(9):3632-42
Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links.
Hicks JK, Chute CL, Paulsen MT, Ragland RL, Howlett NG, Guéranger Q, Glover TW, Canman CE
Molecular and cellular biology 2010 Mar;30(5):1217-30
Molecular and cellular biology 2010 Mar;30(5):1217-30
WRN participates in translesion synthesis pathway through interaction with NBS1.
Kobayashi J, Okui M, Asaithamby A, Burma S, Chen BP, Tanimoto K, Matsuura S, Komatsu K, Chen DJ
Mechanisms of ageing and development 2010 Jun;131(6):436-44
Mechanisms of ageing and development 2010 Jun;131(6):436-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # H00056852-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7. Analysis of RAD18 expression in PC-12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RAD18 Antibody (3H7) [H00056852-M01] - Detection limit for recombinant GST tagged RAD18 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on HeLa cell. Antibody concentration 25 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image was submitted via customer Review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
