A300-390A

antibody from Invitrogen Antibodies

Targeting: DHX15 DBP1, DDX15, HRH2, PRP43, PRPF43, PrPp43p

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A300-390A

Provider

Invitrogen Antibodies

Product name

DHX15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DHX15 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody A300-390A (lot A300-390A-1) used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DHX15 by western blot and immunoprecipitation. Samples: Whole cell lysate (CL; 60 µg for WB, 1 mg for IP) from 293T cells. Antibodies: Affinity purified rabbit anti-DHX15 antibody BL1628 (Cat. No. A300-390A) used at 0.1 µg/ml for WB and at 1 µg/mg lysate for IP. immunoprecipitatesd DHX15 was blotted using affinity purified rabbit anti-DHX15 antibody BL1627 (Cat. No. A300-389A) at 0.1 µg/ml. BL1627 was also used unsuccessfully for IP (1 µg/mg lysate). Detection: Chemiluminescence with an exposure time of 15 seconds.