Explore
Validate
LearnNB100-586
antibody from Novus Biologicals
Targeting: DHX15
DBP1, DDX15, HRH2, PRP43, PRPF43, PrPp43p
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-586 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-586, RRID:AB_2245920
- Product name
- Rabbit Polyclonal DHX15 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-586 is specific for human DHX 15 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Human RNA cap1 methyltransferase CMTr1 cooperates with RNA helicase DHX15 to modify RNAs with highly structured 5' termini.
A weak C' box renders U3 snoRNA levels dependent on hU3-55K binding.
Toczydlowska-Socha D, Zielinska MM, Kurkowska M, Astha, Almeida CF, Stefaniak F, Purta E, Bujnicki JM
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2018 Nov 5;373(1762)
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2018 Nov 5;373(1762)
A weak C' box renders U3 snoRNA levels dependent on hU3-55K binding.
Knox AA, McKeegan KS, Debieux CM, Traynor A, Richardson H, Watkins NJ
Molecular and cellular biology 2011 Jun;31(12):2404-12
Molecular and cellular biology 2011 Jun;31(12):2404-12
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-586. Immunoprecipitated DHX15 was blotted using NB100-585 at 0.1 mcg/ml. NB100-585 was also used unsuccessfully for IP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody NB100-586 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
