LS-C54468

antibody from LSBio

Targeting: AIRE APECED, APS1, PGA1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C54468

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C54468, RRID:AB_2289412

Product name

AIRE Antibody (aa159-172) LS-C54468

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody (0.3 ug/ml) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

AIRE antibody staining (0.05µg/ml) of HEK293 cell lysates. Untransfected (Lane 5 and 7) and transfected with Human AIRE (lane 6) or Mouse AIRE (lane 7). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

AIRE antibody staining (0.05µg/ml) of HEK293 cell lysates. Untransfected (Lane 5 and 7) and transfected with Human AIRE (lane 6) or Mouse AIRE (lane 7). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody (0.3 ug/ml) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.