Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP16040PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP16040PU-N, RRID:AB_1928009
- Product name
- anti AIRE / APECED (Isoform 1+2) (C-term)
- Antibody type
- Polyclonal
- Antigen
- Peptide from C-Terminus of the protein sequence according to NP_000374.1, NP_000649.1.
- Reactivity
- Human
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AIRE Antibody staining (AP16040PU-N at 0.01µg/ml) of HEK293 cell lysate. Lane 1: Untransfected and Lane 2: transfected with Human AIRE. Data kindly provided by Prof. PÌ_rt Peterson, University of Tartu, Estonia.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Staining of Human Lymph lysate using AIRE Antibody Cat.-No AP16040PU-N at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AIRE Antibody (AP16040PU-N at 2.5 µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Paraffin embedded Human Thymus stained with AIRE Antibody Cat.-No AP16040PU-N at 2 µg/ml. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.