Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG63980 - Provider product page
- Provider
- Arigo
- Product name
- anti-AIRE antibody
- Antibody type
- Polyclonal
- Antigen
- C-KDVDLSQPRKGRKP
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Lysates from HEK293 cell untransfected (lane 1, 3, 5) or transfected with human AIRE (lane 2, 4, 6) stained with ARG63485 anti-AIRE antibody (lane 1, 2) or ARG63980 anti-AIRE antibody (lane 3, 4) or ARG64102 anti-AIRE antibody (lane 5, 6) at 0.05 µg/ml dilution. Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4) Data kindly provided by Prof. P?rt Peterson, University stained with ARG63980 anti-AIRE (isoform 1) antibody at 0.05 ?g/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin embedded Human Thymus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63980 anti-AIRE (isoform 1) antibody at 2.5 µg/ml dilution followed by AP-staining.
