R32465

antibody from NSJ Bioreagents

Targeting: AIRE APECED, APS1, PGA1

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32465

Provider

NSJ Bioreagents

Product name

AIRE Antibody

Antibody type

Polyclonal (Antigen purified)

Description

Antigen affinity purified

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

Prior to reconstitution, store at 4°C. After reconstitution, the AIRE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat testis and 2) human HeLa lysate with AIRE antibody at 0.5ug/ml. Expected molecular weight ~58 kDa.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human THP1 cells with AIRE antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AIRE antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HL60 cells with AIRE antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AIRE antibody.