Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 13-9534-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AIRE Monoclonal Antibody (TM-724), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This TM-724 monoclonal antibody reacts with human Autoimmune Regulator (AIRE). This 58 kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). AIRE mRNA in the periphery has been observed in the lymph node, fetal liver, and intestines. Furthermore, expression has been observed in dendritic cells, epithelial cells and PBMCs. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation. As a result of alternative splicing, three isoforms have been reported. The conditions autoimmune-polyendocrinopathy-candiasis-ectodermal-dystrophy (APECED) and autoimmune polyglandular syndrome type 1 (APS-1) arise from mutations in the human AIRE gene. The TM-724 antibody is predicted to recognize all three isoforms of human AIRE (AIRE-1, AIRE-2, AIRE-3). Applications Reported: This TM-724 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunohistochemical staining. Applications Tested: This TM-724 antibody has been tested by immunohistochemistry on FFPE human thymus at less than or equal to 20 µg/mL. Low pH antigen retrieval is optimal although high pH antigen retrieval can also be used. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- TM-724
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Expression and prognostic significance of the autoimmune regulator gene in breast cancer cells.
Bianchi F, Sommariva M, De Cecco L, Triulzi T, Romero-Cordoba S, Tagliabue E, Sfondrini L, Balsari A
Cell cycle (Georgetown, Tex.) 2016 Dec;15(23):3220-3229
Cell cycle (Georgetown, Tex.) 2016 Dec;15(23):3220-3229
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on formalin-fixed paraffin embedded human thymus using 20 µg/mL Rat IgG2a K Isotype Control Biotin (left) or 20 µg/mL Anti-Human AIRE Biotin (right) followed by streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin