Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [2]
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Validation data
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- Product number
- STJ71077 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-AIRE antibody (Internal) (STJ71077)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-AIRE (Internal) is suitable for use in ELISA and Western Blot research applications.
- Reactivity
- Human, Rat, Bovine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
KAKPPKKPESSAEQ
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ71077 (0. 3µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ71077 staining (0. 05µg/ml) of HEK293 cell lysates. Untransfected (Lane 5 and 7) and transfected with Human AIRE (lane 6) or Mouse AIRE (lane 7). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA