RQ4204

antibody from NSJ Bioreagents

Targeting: CD2AP CMS

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4204

Provider

NSJ Bioreagents

Product name

CD2AP Antibody

Antibody type

Polyclonal

Description

This highly specific CD2AP antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry/Immunofluorescence/Immunoprecipitation/ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the CD2AP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) COLO320, 5) 22RV1 6) SK-OV-3, 7) rat stomach, 8) rat liver and 9) mouse liver lysate wtih CD2AP antibody at 0.5ug/ml. Predicted molecular weight: 71-80 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) 293T, 3) MCF7, 4) COLO320, 5) 22RV1 6) SK-OV-3, 7) rat stomach, 8) rat liver and 9) mouse liver lysate wtih CD2AP antibody at 0.5ug/ml. Predicted molecular weight: 71-80 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) Jurkat, 2) A431, 3) HeLa and 4) K562 cell lysate wtih CD2AP antibody. Predicted molecular weight: 71-80 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HeLa cells with CD2AP antibody (green) at 5ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human K562 cells with CD2AP antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunoprecipitation

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunoprecipitation of CD2AP protein from 500ug of human Jurkat whole cell lysate with 2ug of CD2AP antibody.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse kidney tissue with CD2AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat kidney tissue with CD2AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human K562 cells with CD2AP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Caco-2 cells with CD2AP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A431 cells with CD2AP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human K562 cells with CD2AP antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD2AP antibody.