PA5-28391

antibody from Invitrogen Antibodies

Targeting: CD2AP CMS

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28391

Provider

Invitrogen Antibodies

Product name

CD2AP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Rat2, K562, THP-1, HL-60. Predicted reactivity: Mouse (87%), Rat (86%), Pig (92%), Rhesus Monkey (96%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.17 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Upregulation of RIN3 induces endosomal dysfunction in Alzheimer's disease.

Shen R, Zhao X, He L, Ding Y, Xu W, Lin S, Fang S, Yang W, Sung K, Spencer B, Rissman RA, Lei M, Ding J, Wu C
Translational neurodegeneration 2020 Jun 18;9(1):26

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD2AP in paraformaldehyde-fixed A431 cells using a CD2AP polyclonal antibody (Product # PA5-28391) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD2AP Polyclonal Antibody detects CD2AP protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD2AP stained by CD2AP Polyclonal Antibody (Product # PA5-28391) diluted at 1:500. Blue: Fluoroshield with DAPI .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 RIN3 colocalizes with CD2AP and BIN1. PC12M cells ( a and b ) and primary cortical neurons ( c and d ) were transfected with RIN3-GFP, followed by immunostaining for CD2AP ( a , c ) or BIN1 ( b , d ) or CD2AP using specific antibodies. Yellow color denotes colocalization. Representative images are shown

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 BIN1 and CD2AP expression level in APP/PS1 mice. 3-month-old APP/PS1 mice and age-matched WT mice were dissected, lysates were immunoblotted for BIN1 and CD2AP ( a ). The protein levels were normalized against GAPDH as a loading control ( b ). In Cortex ( c ) and hippocampus ( d ) were also extracted from10-month-old APP/PS1 and age-matched WT mice. Protein lysates were used to probe for BIN1, CD2AP, RIN3, Rabex5 and Rab5 using indicated antibodies by SDS-PAGE/immunoblotting ( c and d ), The intensity of bands was quantitated using BioRad-ImageLab. The respective protein levels were normalized against GAPDH as a loading control ( e , f ). p < 0.05 (*), p < 0.01(**), p < 0.0001 (****), standard t-test