- PA5-28391 - Invitrogen Antibodies CD2AP antibody

Submitted references Upregulation of RIN3 induces endosomal dysfunction in Alzheimer's disease.

Shen R, Zhao X, He L, Ding Y, Xu W, Lin S, Fang S, Yang W, Sung K, Spencer B, Rissman RA, Lei M, Ding J, Wu C
Translational neurodegeneration 2020 Jun 18;9(1):26

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD2AP using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate (D) 30 µg HepG2 whole cell lysate and E) 30 µg A375 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD2AP polyclonal antibody (Product # PA5-28391) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CD2AP was performed by separating 30 µg of A431 lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a CD2AP Polyclonal Antibody (Product # PA5-28391) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD2AP Polyclonal Antibody detects CD2AP protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg A375 whole cell lysate/extract.7.5% SDS-PAGE. CD2AP Polyclonal Antibody (Product # PA5-28391) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD2AP Polyclonal Antibody detects CD2AP protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.7.5% SDS-PAGE. CD2AP Polyclonal Antibody (Product # PA5-28391) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CD2-associated protein was achieved by transfecting A-431 with CD2-associated protein-specific siRNAs (Silencer® select Product # S24192, S24191). Western Blot analysis (Fig. a) was performed using Whole-cell extracts from the CD2-associated protein knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2), and untransfected cells (lane 1). The Blot was probed with CD2AP Polyclonal Antibody (Product # PA5-28391, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western Blot is shown in histogram (Fig. b). A decrease in signal upon siRNA mediated knockdown confirms that antibody is specific to CD2-associated protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-CD2AP Polyclonal Antibody (Product # PA5-28391) and a 80 kDa band corresponding to CD2-associated protein was observed across cell lines tested. Whole-cell extracts (30 µg lysate) of A-431 (Lane 1), PC-3 (Lane 2) and K-562 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD2AP in paraformaldehyde-fixed A431 cells using a CD2AP polyclonal antibody (Product # PA5-28391) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD2AP Polyclonal Antibody detects CD2AP protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD2AP stained by CD2AP Polyclonal Antibody (Product # PA5-28391) diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CD2AP Polyclonal Antibody detects CD2AP protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD2AP Polyclonal Antibody (Product # PA5-28391) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using CD2-associated protein (Product # PA5-28391) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 RIN3 colocalizes with CD2AP and BIN1. PC12M cells ( a and b ) and primary cortical neurons ( c and d ) were transfected with RIN3-GFP, followed by immunostaining for CD2AP ( a , c ) or BIN1 ( b , d ) or CD2AP using specific antibodies. Yellow color denotes colocalization. Representative images are shown

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 BIN1 and CD2AP expression level in APP/PS1 mice. 3-month-old APP/PS1 mice and age-matched WT mice were dissected, lysates were immunoblotted for BIN1 and CD2AP ( a ). The protein levels were normalized against GAPDH as a loading control ( b ). In Cortex ( c ) and hippocampus ( d ) were also extracted from10-month-old APP/PS1 and age-matched WT mice. Protein lysates were used to probe for BIN1, CD2AP, RIN3, Rabex5 and Rab5 using indicated antibodies by SDS-PAGE/immunoblotting ( c and d ), The intensity of bands was quantitated using BioRad-ImageLab. The respective protein levels were normalized against GAPDH as a loading control ( e , f ). p < 0.05 (*), p < 0.01(**), p < 0.0001 (****), standard t-test

PA5-28391

Antibody data