AP2163b

antibody from Abcepta

Targeting: TOLLIP IL-1RAcPIP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2163b

Provider

Abcepta

Proper citation

Abgent Cat#AP2163B, RRID:AB_2287705

Product name

TOLLIP Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of TOLLIP (arrow) using rabbit polyclonal TOLLIP Antibody (A236) (Cat. #AP2163b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TOLLIP gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Middle panels, TOLLIP expression in matched normal and tumor breast samples. Here the TOLLIP stain in the invasive ductal carcinoma cells shows a much stronger signal compared with the normal ductal cells. Bottom panels, TOLLIP expression in matched normal and tumor breast samples. In this case, the signal for TOLLIP in the normal ductal cells is stronger than that in the invasive ductal carcinoma cells."

Primary Ab dilution

1:50~100