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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00054472-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00054472-M01, RRID:AB_425988
- Product name
- TOLLIP monoclonal antibody (M01), clone 1A5-2A3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TOLLIP.
- Antigen sequence
MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQL
DAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGM
TRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIH
CTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPE
SLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALL
PAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCS
PGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMD
QEVIRSVLEAQRGNKDAAINSLLQMGEEP- Isotype
- IgG
- Antibody clone number
- 1A5-2A3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TOLLIP monoclonal antibody (M01), clone 1A5-2A3 Western Blot analysis of TOLLIP expression in 293 ( Cat # L026V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TOLLIP expression in transfected 293T cell line by TOLLIP monoclonal antibody (M01), clone 1A5-2A3.Lane 1: TOLLIP transfected lysate (Predicted MW: 30.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TOLLIP is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of TOLLIP transfected lysate using anti-TOLLIP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TOLLIP monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
