ALX-804-132-C100

antibody from Enzo Life Sciences

Targeting: TOLLIP IL-1RAcPIP

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

ALX-804-132-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-132-C100, RRID:AB_2052673

Product name

Tollip monoclonal antibody (Kimmy-1)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Reactivity

Human, Mouse

Host

Rat

Isotype

IgG

Antibody clone number

Kimmy-1

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Threonine 66 in the death domain of IRAK-1 is critical for interaction with signaling molecules but is not a target site for autophosphorylation.

Neumann D, Kollewe C, Pich A, Cao P, Resch K, Martin MU
Journal of leukocyte biology 2008 Sep;84(3):807-13

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Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor.

Burns K, Clatworthy J, Martin L, Martinon F, Plumpton C, Maschera B, Lewis A, Ray K, Tschopp J, Volpe F
Nature cell biology 2000 Jun;2(6):346-51

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Detection of endogenous tollip in murine EL-4. Optimal dilution of Kimmy-1 is between 1-5µg/ml. Extracts should be made from approximately 106 -107 cells. Method: RIPA buffer extracts from 1.5x10E6 EL-4 cells were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with Kimmy-1 at a dilution of 5µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (ALX-211-052) and a chemiluminescence detection system.