Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ALX-804-132-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-132-C100, RRID:AB_2052673
- Product name
- Tollip monoclonal antibody (Kimmy-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- Kimmy-1
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Threonine 66 in the death domain of IRAK-1 is critical for interaction with signaling molecules but is not a target site for autophosphorylation.
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor.
Neumann D, Kollewe C, Pich A, Cao P, Resch K, Martin MU
Journal of leukocyte biology 2008 Sep;84(3):807-13
Journal of leukocyte biology 2008 Sep;84(3):807-13
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor.
Burns K, Clatworthy J, Martin L, Martinon F, Plumpton C, Maschera B, Lewis A, Ray K, Tschopp J, Volpe F
Nature cell biology 2000 Jun;2(6):346-51
Nature cell biology 2000 Jun;2(6):346-51
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Detection of endogenous tollip in murine EL-4. Optimal dilution of Kimmy-1 is between 1-5µg/ml. Extracts should be made from approximately 106 -107 cells. Method: RIPA buffer extracts from 1.5x10E6 EL-4 cells were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with Kimmy-1 at a dilution of 5µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG (ALX-211-052) and a chemiluminescence detection system.