TA500323

antibody from OriGene

Targeting: AK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500323

Provider

OriGene

Proper citation

OriGene Cat#TA500323, RRID:AB_2289415

Product name

AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone OTI18C7 (formerly 18C7)

Antibody type

Monoclonal

Description

AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone OTI18C7 (formerly 18C7)

Host

Mouse

Conjugate

Unconjugated

Epitope

AK1

Isotype

IgG

Antibody clone number

OTI18C7

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-AK1 mouse monoclonal antibody (TA500323) immunofluorescent staining of Hela cells transiently transfected by pCMV6-ENTRY AK1(RC215130).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC215130 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK1 antibody(TA500323), and then analyzed by flow cytometry.

Validation comment

FC