PA5-88402

antibody from Invitrogen Antibodies

Targeting: AK1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88402

Provider

Invitrogen Antibodies

Product name

Adenylate Kinase 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK; Positive Samples: Mouse heart, Mouse skeletal muscle; Cellular Location: Cytoplasm

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.23 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Adenylate Kinase 1 in extracts of various cell lines. Samples were incubated with Adenylate Kinase 1 Polyclonal antibody (Product # PA5-88402) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Adenylate Kinase 1 in HeLa cells. Samples were incubated with Adenylate Kinase 1 Polyclonal antibody (Product # PA5-88402). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Adenylate Kinase 1 in paraffin-embedded human esophageal cancer. Samples were incubated with Adenylate Kinase 1 Polyclonal antibody (Product # PA5-88402) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Adenylate Kinase 1 in paraffin-embedded mouse heart. Samples were incubated with Adenylate Kinase 1 Polyclonal antibody (Product # PA5-88402) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Adenylate Kinase 1 in paraffin-embedded human esophageal cancer. Samples were incubated with Adenylate Kinase 1 Polyclonal antibody (Product # PA5-88402) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Adenylate Kinase 1 in paraffin-embedded mouse heart. Samples were incubated with Adenylate Kinase 1 Polyclonal antibody (Product # PA5-88402) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.