H00000203-M06

antibody from Invitrogen Antibodies

Targeting: AK1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000203-M06

Provider

Invitrogen Antibodies

Product name

AK1 Monoclonal Antibody (3A6-1F5)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: MEEKLKKTNI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3A6-1F5

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AK1 over-expressed 293 cell line, cotransfected with AK1 Validated Chimera RNAi (Cat # H00000203-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AK1 monoclonal antibody (M06), clone 3A6-1F5 (Cat # H00000203-M06). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AK1 expression in transfected 293T cell line by AK1 monoclonal antibody (M06), clone 3A6-1F5.Lane 1: AK1 transfected lysate(21.6 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AK1 monoclonal antibody (M06), clone 3A6-1F5 Western Blot analysis of AK1 expression in HeLa (Cat # L013V1).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AK1 on HeLa cell. Antibody concentration is 10 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of AK1 transfected lysate using anti-AK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AK1 MaxPab rabbit polyclonal antibody.