PA5-28354

antibody from Invitrogen Antibodies

Targeting: AK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28354

Provider

Invitrogen Antibodies

Product name

Adenylate Kinase 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, HCT116, mouse brown adipose tissue. Predicted reactivity: Mouse (89%), Rat (90%), Xenopus laevis (80%), Dog (97%), Chicken (86%), Sheep (96%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Adenylate kinase 1 using 30 µg of HCT116 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Adenylate kinase 1 polyclonal antibody (Product # PA5-28354) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-28354). Sample (50 µg of whole cell lysate). Lane A: Mouse brown adipose tissue. 12% SDS PAGE. Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-28354) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Adenylate Kinase 1 Polyclonal Antibody detects Adenylate kinase 1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. Adenylate Kinase 1 Polyclonal Antibody (Product # PA5-28354) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.