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Western blot
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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA021798 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA021798, RRID:AB_1856911
- Product name
- Anti-SIRT5
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SIRT5, Gene description: sirtuin 5, Validated applications: WB, IHC, Uniprot ID: Q9NXA8, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Repression of p53 function by SIRT5-mediated desuccinylation at Lysine 120 in response to DNA damage
SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer
Liu X, Rong F, Tang J, Zhu C, Chen X, Jia S, Wang Z, Sun X, Deng H, Zha H, Ouyang G, Xiao W
Cell Death & Differentiation 2021;29(4):722-736
Cell Death & Differentiation 2021;29(4):722-736
SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer
Lv X, Liu L, Cheng C, Yu B, Xiong L, Hu K, Tang J, Zeng L, Sang Y
Scientific Reports 2015;5(1)
Scientific Reports 2015;5(1)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and SIRT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415885).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human heart muscle and pancreas tissues using HPA021798 antibody. Corresponding SIRT5 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
