15122-1-AP

antibody from Proteintech Group

Targeting: SIRT5

Provider product page for 15122-1-AP

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References [69]
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Product number: 15122-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15122-1-AP, RRID:AB_2188778
Product name: SIRT5 antibody
Antibody type: Polyclonal
Description: SIRT5 antibody (Cat. #15122-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SIRT5 protein structure - 15122-1-AP shown in red.

Submitted references Acetylation of GPRC5A at Lys348 facilitates cisplatin resistance and promotes the recurrence and poor prognosis in ovarian cancer.

He Q, Qu M, Yang Y, Sun T, Bao H, Shen T, Yao J, Xu C, Wu X, Liu X, Zeng LH
International journal of biological macromolecules 2026 May;362:152113

Sirt3 Genetically Engineered Apoptotic Bodies Alleviate Skeletal Aging by Limiting Aggravated NLRP3 Inflammasome Activation of Senescent Macrophages.

Wu Y, Ling S, Mao J, Wang H, Wang B, Che Z, Cui W, Cai M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 May;13(26):e17554

Sesamin exerts protective effects against diquat-induced acute kidney injury by inhibiting oxidative stress, apoptosis and autophagy: A key role for the SIRT5/FOXO3a signaling pathway.

Han S, Pu Y, Liu Z
Pesticide biochemistry and physiology 2026 Jan;216(Pt 1):106728

The UBC/SIRT5/DRP1 axis regulates mitochondrial dynamics to alleviate Staphylococcus aureus-induced oxidative stress and senescence in bovine mammary epithelial cells.

Hu H, Jiang N, Liu J, Bi J, Liu X, Xu B, Cao Y, Guo W, Fu S
PLoS pathogens 2026 Feb;22(2):e1013975

Targeting TFAM K76 acetylation attenuates mitochondrial dysfunction and kidney injury in diabetic kidney disease.

Fu T, Sun S, Wang Z, Zhao F, Zhen J, Ji X, Xue F, Mu Q, Wang Y, Liu Y, Wan Q
Cardiovascular diabetology 2026 Feb 3;25(1)

Resveratrol Alleviated Oxidative Damage of Bovine Mammary Epithelial Cells via Activating SIRT5-IDH2 Axis.

Yang H, Liu L, Zhang X, Gao S, Li A, Dong J, Lu G, Yang Q, Liu X, Sun S, Li H, Liu Y, Wang Y, Han Y
Antioxidants (Basel, Switzerland) 2025 Sep 26;14(10)

SIRT5 is associated with asthenozoospermia and regulates GC‑2 spd cell proliferation and apoptosis.

Xing H, Chen S, Wang M, Zheng T, Yang B
Molecular medicine reports 2025 Oct;32(4)

SIRT5-mediated desuccinylation of MTHFD2 enhances chemoresistance in breast cancer cells by reducing therapy-induced senescence.

Xianhong Z, Yue G, Yu Z, Yichen Z, Yufei C, Xinke P, Jinhui Z, Yiqi W, Yitian D, Shuailin H, Ting N
Communications biology 2025 Oct 20;8(1):1485

SIRT5 modulates mitochondria function via mitophagy and antioxidant mechanisms to facilitate oocyte maturation in mice.

Yan JJ, Wang YY, Shi ZY, Ding YY, Wen HQ, Wu MP, Sun SC, Cai YF, Zhang Y
International journal of biological macromolecules 2025 May;306(Pt 2):141488

Sirt5-mediated polarization and metabolic reprogramming of macrophage sustain brain function following ischemic stroke.

Zhang L, Lv T, Hou P, Jin Y, Jia F
Brain research 2025 Jun 15;1857:149613

The SIRT6/BAP1/xCT signaling axis mediates ferroptosis in cisplatin-induced AKI.

Yang S, Chen L, Din S, Ye Z, Zhou X, Cheng F, Li W
Cellular signalling 2025 Jan;125:111479

l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function.

Liu S, Jin Y, Zhou W, Liu J, Tu L, Zhang Y, Zhang M, Liu X, Cao Y, Guo W, Fu S
Journal of agricultural and food chemistry 2025 Feb 12;73(6):3483-3493

SIRT5 regulates fatty acid oxidation and mitochondrial oxidative stress to exacerbate airway inflammation in experimental asthma.

Wu J, Wang Y, Zhang Q, Lan Y, Chen M, Wang J, Zhao X, Chen Y, Peng X, Zhao W, Zhao H
Cellular signalling 2025 Dec;136:112149

Sirtuin 3-mediated delactylation of malic enzyme 2 disrupts redox balance and inhibits colorectal cancer growth.

Li C, Ge C, Wang Q, Teng P, Jia H, Yao S, Huang Z
Cellular oncology (Dordrecht, Netherlands) 2025 Aug;48(4):979-990

Sirtuin 1-mediated recruitment of non-structural protein 1 to glucocorticoid receptor induces glucocorticoid insensitivity post RSV infection.

Che S, Long X, Mo S, Jiang Z, Xie X, Liu E, Xie J
International journal of biological macromolecules 2025 Aug;320(Pt 1):145811

MRPL12 K163 acetylation inhibits ccRCC via driving mitochondrial metabolic reprogramming.

Ji X, Xue F, Wang Y, Gao D, Sun J, Dong T, Mu Q, Xu Q, Sun S, Liu Y, Wan Q
Cell death & disease 2025 Aug 26;16(1):646

Semaglutide protects against diabetes-associated cardiac inflammation via Sirt3-dependent RKIP pathway.

Lin K, Wang A, Zhai C, Zhao Y, Hu H, Huang D, Zhai Q, Yan Y, Ge J
British journal of pharmacology 2025 Apr;182(7):1561-1581

SIRT5-mediated BCAT1 desuccinylation and stabilization leads to ferroptosis insensitivity and promotes cell proliferation in glioma.

Wang T, Han XH, Chen JJ, Wang X, Zhang Z, Han XJ, Lu Z
Cell death & disease 2025 Apr 7;16(1):261

Astragaloside IV protects renal tubular epithelial cells against oxidative stress-induced injury by upregulating CPT1A-mediated HSD17B10 lysine succinylation in diabetic kidney disease.

Wang M, Li Q, Wang S, Zuo L, Hai Y, Yuan S, Li X, Huang X, Yang C, Yao L, Cao W, Zuo G, Wang J
Phytotherapy research : PTR 2024 Sep;38(9):4519-4540

Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation.

Zhou J, Zhang L, Peng J, Zhang X, Zhang F, Wu Y, Huang A, Du F, Liao Y, He Y, Xie Y, Gu L, Kuang C, Ou W, Xie M, Tu T, Pang J, Zhang D, Guo K, Feng Y, Yin S, Cao Y, Li T, Jiang Y
Cell metabolism 2024 Sep 3;36(9):2054-2068.e14

Nucleolin malonylation as a nuclear-cytosol signal exchange mechanism to drive cell proliferation in Hepatocarcinoma by enhancing AKT translation.

Sun L, Meng H, Liu T, Zhao Q, Xia M, Zhao Z, Qian Y, Cui H, Zhong X, Chai K, Tian Y, Sun Y, Zhu B, Di J, Shui G, Zhang L, Zheng J, Guo S, Liu Y
The Journal of biological chemistry 2024 Oct;300(10):107785

Tetrandrine targeting SIRT5 exerts anti-melanoma properties via inducing ROS, ER stress, and blocked autophagy.

Ji Y, Li C, Wan S, Dong Z, Liu C, Guo L, Shi S, Ci M, Xu M, Li Q, Hu H, Cui H, Liu Y
Journal of pharmaceutical analysis 2024 Oct;14(10):101036

Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury.

Yu Q, Zhang J, Li J, Song Y, Pan J, Mei C, Cui M, He Q, Wang H, Li H, Cheng B, Zhang Y, Guo W, Zhu C, Chen S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Oct;11(39):e2402710

Spatial distribution of maternal factors in pig mature oocytes.

Zhu F, Yang M, Wang D, Jiang Y, Jia C, Fu Y, Yu A, Liu H, Wang M, Wang T, Liu H, Li J
Animal biotechnology 2024 Nov;35(1):2394692

The Sirtuin 5 Inhibitor MC3482 Ameliorates Microglia‑induced Neuroinflammation Following Ischaemic Stroke by Upregulating the Succinylation Level of Annexin-A1.

Xia Q, Yu Y, Zhan G, Zhang X, Gao S, Han T, Zhao Y, Li X, Wang Y
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2024 May 8;19(1):17

The SP1/SIRT1/ACLY signaling axis mediates fatty acid oxidation in renal ischemia-reperfusion-induced renal fibrosis.

Wu H, Wang L, Kang P, Zhou X, Li W, Xia Z
International immunopharmacology 2024 May 10;132:112002

Corn Oligopeptide Alleviates Nonalcoholic Fatty Liver Disease by Regulating the Sirtuin Signaling Pathway.

Xu Y, Su T, Mishra H, Ando R, Furutani Y, Lu J, Cai M, Suzuki H, Yu W, Qin XY
Journal of agricultural and food chemistry 2024 Mar 27;72(12):6360-6371

Comprehensive pan-cancer analysis reveals SIRT5 is a predictive biomarker for prognosis and immunotherapy response.

Ji Y, Li C, Wan S, Zhang K, Liu Y, Shi S
Functional & integrative genomics 2024 Mar 19;24(2):60

Mitochondrial glycerol 3-phosphate dehydrogenase deficiency exacerbates lipotoxic cardiomyopathy.

Qu H, Liu X, Zhu J, He N, He Q, Zhang L, Wang Y, Gong X, Xiong X, Liu J, Wang C, Yang G, Yang Q, Luo G, Zhu Z, Zheng Y, Zheng H
iScience 2024 Jun 21;27(6):109796

SIRT5-mediated ME2 desuccinylation promotes cancer growth by enhancing mitochondrial respiration.

Teng P, Cui K, Yao S, Fei B, Ling F, Li C, Huang Z
Cell death and differentiation 2024 Jan;31(1):65-77

Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma.

Liang J, Zhou C, Zhang C, Liang S, Zhou Z, Zhou Z, Wu C, Zhao H, Meng X, Zou F, Yu C, Cai S
International immunopharmacology 2024 Jan 25;127:111328

Sirt6 regulates the proliferation of neural precursor cells and cortical neurogenesis in mice.

Wei Y, Wang X, Ma Z, Xiang P, Liu G, Yin B, Hou L, Shu P, Liu W, Peng X
iScience 2024 Feb 16;27(2):108706

Serine synthesis sustains macrophage IL-1β production via NAD(+)-dependent protein acetylation.

Wang C, Chen Q, Chen S, Fan L, Gan Z, Zhao M, Shi L, Bin P, Yang G, Zhou X, Ren W
Molecular cell 2024 Feb 15;84(4):744-759.e6

Antitumorigenic potential of Lactobacillus-derived extracellular vesicles: p53 succinylation and glycolytic reprogramming in intestinal epithelial cells via SIRT5 modulation.

Zhang J, Huang X, Zhang T, Gu C, Zuo W, Fu L, Dong Y, Liu H
Cell biology and toxicology 2024 Aug 7;40(1):66

Oxidative stress and regulation of adipogenic differentiation capacity by sirtuins in adipose stem cells derived from female patients of advancing age.

Bernhardt A, Jamil A, Morshed MT, Ponnath P, Gille V, Stephan N, Sauer H, Wartenberg M
Scientific reports 2024 Aug 27;14(1):19885

SIRT5 Regulates Ferroptosis through the Nrf2/HO-1 Signaling Axis to Participate in Ischemia-Reperfusion Injury in Ischemic Stroke.

Li J, Wei G, Song Z, Chen Z, Gu J, Zhang L, Wang Z
Neurochemical research 2024 Apr;49(4):998-1007

Prognostic value of sirtuin family members and experimental verification identify SIRT5 as diagnostic biomarkers in clear cell renal cell carcinoma.

Peng LS, Duan SL, Li RQ, Bai ZY, Ou CL, Wang JP
PeerJ 2023;11:e15154

ASPSCR-1 and Sirt-5 alleviate Clonorchis liver fluke rCsNOSIP-induced oxidative stress, proliferation, and migration in cholangiocarcinoma cells.

Bian M, Li S, Zhou H, Bi L, Shen Y, Tingjin C, Yu X, Huang Y, Xu Q
PLoS neglected tropical diseases 2023 Nov;17(11):e0011727

LncRNA GLTC targets LDHA for succinylation and enzymatic activity to promote progression and radioiodine resistance in papillary thyroid cancer.

Shi L, Duan R, Sun Z, Jia Q, Wu W, Wang F, Liu J, Zhang H, Xue X
Cell death and differentiation 2023 Jun;30(6):1517-1532

Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain.

Zuo CY, Gou CY, Zhang CS, Zhou X, Lv P, Zhang HX, Fan ZP, Tian FW, Wang ZX
Heliyon 2023 Jul;9(7):e17765

Mao J, Wang D, Wang D, Wu Q, Shang Q, Gao C, Wang H, Wang H, Du M, Peng P, Jia H, Xu X, Wang J, Yang L, Luo Z
Experimental & molecular medicine 2023 Jan;55(1):253-268

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice.

Chen JW, Shan TK, Wei TW, Jiang QQ, Du C, Gu LF, Yang TT, Zhou LH, Wang SB, Bao YL, Wang H, Ji Y, Xie LP, Gu AH, Sun CQ, Wang QM, Wang LS
Archives of toxicology 2023 Dec;97(12):3209-3226

A cell-penetrating peptide exerts therapeutic effects against ischemic stroke by mediating the lysosomal degradation of sirtuin 5.

Xia Q, Zhang X, Zhan G, Zheng L, Mao M, Zhao Y, Zhao Y, Li X
MedComm 2023 Dec;4(6):e436

Inhibition of integrin receptors reduces extracellular matrix levels, ameliorating benign prostate hyperplasia.

Yang C, Zhao J, Lin C, Gao Y, Luo J, He F, Fang Z, Li Z, Ran Q, Yang Z
International journal of biological macromolecules 2023 Dec 31;253(Pt 1):126499

Alzheimer's Amyloid-β Accelerates Human Neuronal Cell Senescence Which Could Be Rescued by Sirtuin-1 and Aspirin.

Li Y, Lu J, Hou Y, Huang S, Pei G
Frontiers in cellular neuroscience 2022;16:906270

Constructing a signature based on the SIRT family to help the prognosis and treatment sensitivity in glioma patients.

Xuan F, Zhang Z, Liu K, Gong H, Liang S, Zhao Y, Li H
Frontiers in genetics 2022;13:1035368

Omentin1 ameliorates myocardial ischemia-induced heart failure via SIRT3/FOXO3a-dependent mitochondrial dynamical homeostasis and mitophagy.

Hu J, Liu T, Fu F, Cui Z, Lai Q, Zhang Y, Yu B, Liu F, Kou J, Li F
Journal of translational medicine 2022 Oct 4;20(1):447

Magnesium Isoglycyrrhizinate Reduces the Target-Binding Amount of Cisplatin to Mitochondrial DNA and Renal Injury through SIRT3.

Wang X, Zhu H, Hu J, Li H, Guo S, Chen B, Liu C, Wang G, Zhou F
International journal of molecular sciences 2022 Oct 28;23(21)

Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin.

Head PE, Myung S, Chen Y, Schneller JL, Wang C, Duncan N, Hoffman P, Chang D, Gebremariam A, Gucek M, Manoli I, Venditti CP
Science translational medicine 2022 May 25;14(646):eabn4772

Sirtuin 5 aggravates microglia-induced neuroinflammation following ischaemic stroke by modulating the desuccinylation of Annexin-A1.

Xia Q, Gao S, Han T, Mao M, Zhan G, Wang Y, Li X
Journal of neuroinflammation 2022 Dec 14;19(1):301

Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism.

Zhou B, Xiao M, Hu H, Pei X, Xue Y, Miao G, Wang J, Li W, Du Y, Zhang P, Wei T
Frontiers in cell and developmental biology 2021;9:687559

Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome.

Peoples JN, Ghazal N, Duong DM, Hardin KR, Manning JR, Seyfried NT, Faundez V, Kwong JQ
American journal of physiology. Cell physiology 2021 Sep 1;321(3):C519-C534

Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.

Sivalingam K, Doke M, Khan MA, Samikkannu T
Scientific reports 2021 Oct 29;11(1):21335

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Qu H, Gong X, Liu X, Zhang R, Wang Y, Huang B, Zhang L, Zheng H, Zheng Y
Diabetes 2021 Jun;70(6):1372-1387

20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.

Cui W, Wu X, Shi Y, Guo W, Luo J, Liu H, Zheng L, Du Y, Wang P, Wang Q, Feng D, Ge S, Qu Y
Cell proliferation 2021 Feb;54(2):e12964

Proteomic analysis of lysine acetylation reveals that metabolic enzymes and heat shock proteins may be potential targets for DSS-induced mice colitis.

Wang JM, Lin SR, Zhu YB, Yuan J, Wang YM, Zhang Q, Xie LS, Li SH, Liu SQ, Yu SG, Wu QF
International immunopharmacology 2021 Dec;101(Pt B):108336

SIRT4 is the molecular switch mediating cellular proliferation in colorectal cancer through GLS mediated activation of AKT/GSK3β/CyclinD1 pathway.

Cui Y, Bai Y, Yang J, Yao Y, Zhang C, Liu C, Shi J, Li Q, Zhang J, Lu X, Zhang Y
Carcinogenesis 2021 Apr 17;42(3):481-492

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Li ST, Huang D, Shen S, Cai Y, Xing S, Wu G, Jiang Z, Hao Y, Yuan M, Wang N, Zhu L, Yan R, Yang D, Wang L, Liu Z, Hu X, Zhou R, Qu K, Li A, Duan X, Zhang H, Gao P
Nature metabolism 2020 Mar;2(3):256-269

Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy.

Ali HR, Michel CR, Lin YH, McKinsey TA, Jeong MY, Ambardekar AV, Cleveland JC, Reisdorph R, Reisdorph N, Woulfe KC, Fritz KS
Journal of molecular and cellular cardiology 2020 Jan;138:304-317

Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1.

Guan J, Jiang X, Gai J, Sun X, Zhao J, Li J, Li Y, Cheng M, Du T, Fu L, Li Q
Journal of cellular and molecular medicine 2020 Dec;24(23):14039-14049

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Fu Y, Sun Y, Wang M, Hou Y, Huang W, Zhou D, Wang Z, Yang S, Tang W, Zhen J, Li Y, Wang X, Liu M, Zhang Y, Wang B, Liu G, Yu X, Sun J, Zhang C, Yi F
Cell metabolism 2020 Dec 1;32(6):1052-1062.e8

Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer.

Sun X, Wang S, Li Q
Frontiers in genetics 2019;10:879

Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7.

Fan X, Yan K, Meng Q, Sun R, Yang X, Yuan D, Li F, Deng H
International journal of molecular medicine 2019 Jul;44(1):157-171

Lysine glutarylation in human sperm is associated with progressive motility.

Cheng YM, Hu XN, Peng Z, Pan TT, Wang F, Chen HY, Chen WQ, Zhang Y, Zeng XH, Luo T
Human reproduction (Oxford, England) 2019 Jul 8;34(7):1186-1194

SIRT5-mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer.

Shi L, Yan H, An S, Shen M, Jia W, Zhang R, Zhao L, Huang G, Liu J
Molecular oncology 2019 Feb;13(2):358-375

The DNA Endonuclease Mus81 Regulates ZEB1 Expression and Serves as a Target of BET4 Inhibitors in Gastric Cancer.

Yin Y, Liu W, Shen Q, Zhang P, Wang L, Tao R, Li H, Ma X, Zeng X, Cheong JH, Song S, Ajani JA, Mills GB, Tao K, Peng G
Molecular cancer therapeutics 2019 Aug;18(8):1439-1450

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Ma Y, Qi Y, Wang L, Zheng Z, Zhang Y, Zheng J
Free radical biology & medicine 2019 Apr;134:458-467

SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.

Du Y, Hu H, Qu S, Wang J, Hua C, Zhang J, Wei P, He X, Hao J, Liu P, Yang F, Li T, Wei T
EBioMedicine 2018 Oct;36:347-357

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Liu M, Liang K, Zhen J, Zhou M, Wang X, Wang Z, Wei X, Zhang Y, Sun Y, Zhou Z, Su H, Zhang C, Li N, Gao C, Peng J, Yi F
Nature communications 2017 Sep 4;8(1):413

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Submitted by: Proteintech Group (provider)
Main image
Experimental details: The SIRT5 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SIRT5. This antibody recognizes human, mouse, rat antigen. The SIRT5 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 15122-1-AP.pdf

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