Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 698801 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#698801, RRID:AB_2687321
- Product name
- Purified anti-SIRT5
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- O91G9
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate (15µg protein) from THP-1, HeLa and Raw264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 (2µg/mL), 1:1000 (0.5µg/mL) and 1:2500 (0.2µg/mL) diluted purified anti-SIRT5 antibody (clone O91G9) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody for clone O91G9 or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder, M* indicates longer exposure.
- Conjugate
- Unconjugated