698802

antibody from BioLegend

Targeting: SIRT5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

698802

Provider

BioLegend

Proper citation

BioLegend Cat#698802, RRID:AB_2687322

Product name

Purified anti-SIRT5

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

O91G9

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate (15µg protein) from THP-1, HeLa and Raw264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 (2µg/mL), 1:1000 (0.5µg/mL) and 1:2500 (0.2µg/mL) diluted purified anti-SIRT5 antibody (clone O91G9) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody for clone O91G9 or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder, M* indicates longer exposure.

Conjugate

Unconjugated