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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503281 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503281, RRID:AB_11141348
- Product name
- SIRT5 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Antibody type
- Monoclonal
- Description
- SIRT5 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SIRT5
- Isotype
- IgG
- Antibody clone number
- OTI1A6
- Vial size
- 100 µl
- Concentration
- 1.1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-SIRT5 mouse monoclonal antibody (TA503281).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SIRT5 mouse monoclonal antibody (TA503281) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200189 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SIRT5 antibody(TA503281), and then analyzed by flow cytometry.
- Validation comment
- FC
